SgfI, a new type-II restriction endonuclease that recognizes the octanucleotide sequence 5'-GCGAT/CGC-3'

J R Kappelman; M Brady; K Knoche; E Murray; T Schoenfeld; R Williams; N Vesselinova

doi:10.1016/0378-1119(95)00197-e

SgfI, a new type-II restriction endonuclease that recognizes the octanucleotide sequence 5'-GCGAT/CGC-3'

Gene. 1995 Jul 4;160(1):55-8. doi: 10.1016/0378-1119(95)00197-e.

Authors

J R Kappelman¹, M Brady, K Knoche, E Murray, T Schoenfeld, R Williams, N Vesselinova

Affiliation

¹ Promega Corp., Madison, WI 53711-5399, USA.

PMID: 7628716
DOI: 10.1016/0378-1119(95)00197-e

Abstract

A new restriction endonuclease (ENase), SgfI, has been isolated from the bacterium Streptomyces sp. SgfI recognizes the 8-bp palindrome 5'-GCGATCGC-3' and cleaves double-stranded DNA after the T in this sequence, producing a two-base 3' overhang compatible with PvuI termini. SgfI is a rare-cutting ENase and should be useful for megabase mapping experiments.

MeSH terms

Base Sequence
DNA / chemistry
DNA / metabolism
Deoxyribonucleases, Type II Site-Specific / isolation & purification
Deoxyribonucleases, Type II Site-Specific / metabolism*
Kinetics
Methylation
Molecular Sequence Data
Oligodeoxyribonucleotides / metabolism*
Streptomyces / enzymology*
Substrate Specificity

Substances

Oligodeoxyribonucleotides
DNA
endodeoxyribonuclease PvuI
endodeoxyribonuclease SgfI
Deoxyribonucleases, Type II Site-Specific