SgfI, a new type-II restriction endonuclease that recognizes the octanucleotide sequence 5'-GCGAT/CGC-3'

Gene. 1995 Jul 4;160(1):55-8. doi: 10.1016/0378-1119(95)00197-e.


A new restriction endonuclease (ENase), SgfI, has been isolated from the bacterium Streptomyces sp. SgfI recognizes the 8-bp palindrome 5'-GCGATCGC-3' and cleaves double-stranded DNA after the T in this sequence, producing a two-base 3' overhang compatible with PvuI termini. SgfI is a rare-cutting ENase and should be useful for megabase mapping experiments.

MeSH terms

  • Base Sequence
  • DNA / chemistry
  • DNA / metabolism
  • Deoxyribonucleases, Type II Site-Specific / isolation & purification
  • Deoxyribonucleases, Type II Site-Specific / metabolism*
  • Kinetics
  • Methylation
  • Molecular Sequence Data
  • Oligodeoxyribonucleotides / metabolism*
  • Streptomyces / enzymology*
  • Substrate Specificity


  • Oligodeoxyribonucleotides
  • DNA
  • endodeoxyribonuclease PvuI
  • endodeoxyribonuclease SgfI
  • Deoxyribonucleases, Type II Site-Specific