Cloning and characterisation of a nuclear, site specific ssDNA binding protein

Nucleic Acids Res. 1995 Jul 11;23(13):2389-95. doi: 10.1093/nar/23.13.2389.


Estradiol inducible, liver-specific expression of the apoVLDL II gene is mediated through the estrogen receptor and a variety of other DNA-binding proteins. In the present study we report the cloning and characterisation of a single-strand DNA binding protein that interacts with the lower strand of a complex regulatory site, which includes the major estrogen responsive element and a site that resembles the rat albumin site D (apoVLDL II site D). Based on its binding specificity determined with electro-mobility shift assays, the protein is named single-strand D-box binding factor (ssDBF). Analysis of the deduced 302 amino acid sequence revealed that the protein belongs to the heteronuclear ribonucleoprotein A/B family (hnRNP A/B) and resembles other known eukaryotic single-strand DNA binding proteins. Transient transfection experiments in a chicken liver cell-line showed that the protein represses estrogen-induced transcription. A protein with similar binding characteristics is present in liver nuclear extract. The relevance of the occurrence of this protein to the expression of the apoVLDL II gene is discussed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Binding Sites
  • Chickens
  • Cloning, Molecular*
  • DNA-Binding Proteins / chemistry
  • DNA-Binding Proteins / genetics*
  • DNA-Binding Proteins / metabolism
  • Escherichia coli
  • Gene Expression
  • Gene Transfer Techniques
  • Lipoproteins, VLDL / genetics
  • Liver / chemistry
  • Male
  • Molecular Sequence Data
  • RNA, Messenger / analysis
  • Recombinant Fusion Proteins
  • Regulatory Sequences, Nucleic Acid
  • Ribonucleoproteins / chemistry
  • Ribonucleoproteins / genetics*
  • Ribonucleoproteins / metabolism


  • DNA-Binding Proteins
  • Lipoproteins, VLDL
  • RNA, Messenger
  • Recombinant Fusion Proteins
  • Ribonucleoproteins
  • single-strand D-box binding protein, Gallus gallus

Associated data

  • GENBANK/X80340