Advantage of a rapid extraction method of HIV1 DNA suitable for polymerase chain reaction

Res Virol. Mar-Apr 1995;146(2):159-62. doi: 10.1016/0923-2516(96)81085-5.

Abstract

We describe a new protocol for extraction of DNA suitable for HIV1 gene amplification from clinical samples using "Chelex-100" chelating resin. Comparison was made with the classic proteinase K extraction method; 154 specimens were extracted with both methods and subjected to PCR (polymerase chain reaction). The Chelex-100 procedure optimized the yield of DNA recovery and minimized contamination due to sample manipulation. It decreased false negative results due to PCR inhibitors. A DNA sample suitable for use in PCR was obtained in 30 minutes. Chelex-100 treatment is a simple, rapid and low-cost method for DNA extraction in clinical laboratories.

Publication types

  • Clinical Trial
  • Comparative Study

MeSH terms

  • Cells, Cultured
  • Chelating Agents
  • DNA, Viral / isolation & purification*
  • Endopeptidase K
  • HIV Infections / blood
  • HIV Infections / cerebrospinal fluid
  • HIV Infections / virology*
  • HIV-1 / genetics
  • HIV-1 / isolation & purification*
  • Humans
  • Leukocytes, Mononuclear / virology
  • Polymerase Chain Reaction / methods*
  • Resins, Synthetic
  • Serine Endopeptidases

Substances

  • Chelating Agents
  • DNA, Viral
  • Resins, Synthetic
  • Chelex 100
  • Serine Endopeptidases
  • Endopeptidase K