Abstract
The platelet-derived growth factor beta-receptor undergoes polyubiquitination as a consequence of ligand binding. In the present study, we have examined the ligand-induced receptor ubiquitination also in the other receptor tyrosine kinase (structurally different) subfamilies by immunoblotting with anti-ubiquitin antiserum. In addition to the platelet-derived growth factor alpha- and beta-receptors, all the monomeric receptor tyrosine kinases examined, such as the receptors for epidermal growth factor (subfamily I), colony stimulating factor-1 (subfamily III), and fibroblast growth factor (subfamily IV), were found to be ubiquitinated after ligand stimulation. However, the insulin receptor (subfamily II), which is a tetrameric molecule, was not. These data suggest that the ligand-induced polyubiquitination of the receptor is a general phenomenon observed in most of the monomeric receptor tyrosine kinases.
MeSH terms
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3T3 Cells
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Animals
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Aorta
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Becaplermin
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CHO Cells
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Cells, Cultured
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Cricetinae
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Endothelium, Vascular / metabolism*
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ErbB Receptors / isolation & purification
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ErbB Receptors / metabolism
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Humans
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Immunoblotting
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Kinetics
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Macromolecular Substances
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Male
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Mice
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Platelet-Derived Growth Factor / pharmacology*
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Proto-Oncogene Proteins c-sis
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Receptor Protein-Tyrosine Kinases / drug effects
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Receptor Protein-Tyrosine Kinases / metabolism*
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Receptor, Insulin / isolation & purification
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Receptor, Insulin / metabolism
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Receptor, Macrophage Colony-Stimulating Factor / isolation & purification
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Receptor, Macrophage Colony-Stimulating Factor / metabolism
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Receptors, Fibroblast Growth Factor / isolation & purification
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Receptors, Fibroblast Growth Factor / metabolism
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Receptors, Platelet-Derived Growth Factor / isolation & purification
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Receptors, Platelet-Derived Growth Factor / metabolism*
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Recombinant Proteins / isolation & purification
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Recombinant Proteins / metabolism
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Recombinant Proteins / pharmacology
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Skin / metabolism
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Swine
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Transfection
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Ubiquitins / metabolism*
Substances
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Macromolecular Substances
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Platelet-Derived Growth Factor
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Proto-Oncogene Proteins c-sis
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Receptors, Fibroblast Growth Factor
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Recombinant Proteins
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Ubiquitins
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Becaplermin
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ErbB Receptors
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Receptor Protein-Tyrosine Kinases
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Receptor, Insulin
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Receptor, Macrophage Colony-Stimulating Factor
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Receptors, Platelet-Derived Growth Factor