Serum interleukin-8 (IL-8) is elevated in patients with metastatic melanoma and correlates with tumour load

Melanoma Res. 1995 Jun;5(3):179-81. doi: 10.1097/00008390-199506000-00006.


It was recently demonstrated that IL-8 is produced by melanoma cell lines and acts as an essential autocrine growth factor. We studied the constitutive production of IL-8 by melanoma cell lines and the serum concentrations in patients with metastatic melanoma. All of 10 melanoma cell lines investigated constitutively produced IL-8 (mean 315 +/- 58 pg/10(5) cells per 24 h. IL-8 was detectable (mean 159 +/- 13.1 pg/ml) in the serum of 21 out of 56 patients by an enzyme-linked immunosorbent assay (ELISA; detection limit < 100 pg/ml). There was a significant correlation with tumour load, whereas no correlation with metastatic sites was found. No increased IL-8 levels were seen in healthy controls or patients with metastatic renal cell carcinoma. These results suggest that IL-8 is constitutively produced by melanoma cells in vivo.

MeSH terms

  • Cytokines / pharmacology
  • Enzyme-Linked Immunosorbent Assay
  • Growth Substances
  • Humans
  • Interferon-alpha / pharmacology
  • Interferon-gamma / pharmacology
  • Interleukin-8 / biosynthesis
  • Interleukin-8 / blood*
  • Melanoma / blood
  • Melanoma / metabolism*
  • Melanoma / pathology
  • Melanoma / secondary*
  • Tumor Cells, Cultured
  • Tumor Necrosis Factor-alpha / pharmacology


  • Cytokines
  • Growth Substances
  • Interferon-alpha
  • Interleukin-8
  • Tumor Necrosis Factor-alpha
  • Interferon-gamma