Molecular cloning and functional expression of a novel CC chemokine receptor cDNA from a human basophilic cell line

J Biol Chem. 1995 Aug 18;270(33):19495-500. doi: 10.1074/jbc.270.33.19495.


We report the cloning and characterization of a novel basophil CC chemokin receptor, K5-5, from the human immature basophilic cell line KU-812. The predicted protein sequence of K5-5 shows only 49% identity to the macrophage inflammatory protein-1 alpha/RANTES receptor (CC CKR-1) and 47% identity to monocyte chemotactic protein-1 receptor (b form), suggesting that this cDNA encodes a novel member of the CC chemokine receptor family. Analysis of K5-5 mRNA expression indicates that it is restricted to leukocyte-rich tissues. In addition, we have shown significant levels of K5-5 mRNA in human basophils, which were up-regulated by treatment with interleukin-5. The CC chemokines, Macrophage inflammatory protein-1 alpha, RANTES, and monocyte chemotactic protein-1 were able to stimulate a Ca(2+)-activated chloride channel in Xenopus laevis oocytes injected with K5-5 cRNA, whereas no signal was detected in response to monocyte chemotactic protein-2, macrophage inflammatory protein-1 beta, or the CXC chemokine, interleukin-8. Taken together, these results indicate for the first time the presence of a CC chemokine receptor on basophils, which functions as a "shared" CC chemokine receptor and may therefore be implicated in the pathogenesis of basophil-mediated allergic diseases.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Basophils / cytology
  • Basophils / metabolism*
  • Cell Line
  • Chemotaxis, Leukocyte
  • Cloning, Molecular
  • DNA, Complementary
  • Humans
  • Molecular Sequence Data
  • Receptors, CCR4
  • Receptors, Chemokine*
  • Receptors, Cytokine / genetics*
  • Sequence Homology, Amino Acid
  • Xenopus laevis


  • CCR4 protein, human
  • DNA, Complementary
  • Receptors, CCR4
  • Receptors, Chemokine
  • Receptors, Cytokine

Associated data

  • GENBANK/X85740