Sequence-specific DNA binding activities of nuclear matrix proteins of mammalian lens epithelial cells

J Cell Biochem. 1995 May;58(1):1-5. doi: 10.1002/jcb.240580102.

Abstract

This study examines matrix and nonmatrix nuclear proteins of the rabbit lens epithelial cells. The nuclear matrix proteins were isolated by modified Penman technique, which requires presence of detergents and nucleases, whereas nonmatrix nuclear proteins were obtained by high salt extraction. The data from these experiments revealed presence of DNA binding activities for SP-1 and OCT-1 proteins in both matrix and non-matrix compartments of rabbit lens epithelial cells. Comparison of the relative abundance of SP-1 and OCT-1 binding activities in nuclear matrix and nonmatrix fractions suggest the distribution between these two compartments is cell type specific and possibly related to the control of cell growth.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antigens, Nuclear
  • Base Sequence
  • Cells, Cultured
  • DNA-Binding Proteins / metabolism*
  • Epithelium / metabolism
  • Humans
  • Lens, Crystalline / metabolism*
  • Male
  • Molecular Sequence Data
  • Nuclear Proteins / metabolism*
  • Promoter Regions, Genetic*
  • Rabbits
  • Transcription Factors / metabolism

Substances

  • Antigens, Nuclear
  • DNA-Binding Proteins
  • Nuclear Proteins
  • Transcription Factors