Interferon-beta downregulates expression of VLA-4 antigen and antagonizes interferon-gamma-induced expression of HLA-DQ on human peripheral blood monocytes

J Neuroimmunol. 1995 Jul;60(1-2):99-106. doi: 10.1016/0165-5728(95)00059-b.


We have studied the effect of recombinant human IFN-beta on the basal and IFN-gamma-induced expression of adhesion molecules and class II MHC antigens on human peripheral blood monocytes and on ICAM-1 (intercellular adhesion molecule-1) expression of a human umbilical vein endothelial cell line (EAhy 926). We show that IFN-beta downregulates both basal and IFN-gamma-induced expression of VLA-4 (very late activation antigen-4) antigen on monocytes, but has no effect on the expression of CD11a, CD11b, CD11c, L-selectin, CD18, ICAM-1, beta 1-integrin or CD44 on monocytes or ICAM-1 on EAhy 926 cells. We also show that IFN-beta antagonizes the IFN-gamma-induced expression of HLA-DQ-antigen, but not HLA-DR or HLA-DP antigens on monocyte surface. These findings may partially explain the beneficial effect of IFN-beta in multiple sclerosis, since VLA-4-antigen is critical for leukocyte recruitment into inflamed brain and downregulation of HLA-class II expression diminishes antigen presenting capacity of monocytes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cells, Cultured
  • Drug Combinations
  • Endothelium, Vascular / cytology
  • Endothelium, Vascular / metabolism
  • HLA-DQ Antigens / immunology*
  • Histocompatibility Antigens Class II / immunology
  • Humans
  • Integrin alpha Chains*
  • Intercellular Adhesion Molecule-1 / metabolism
  • Interferon-beta / pharmacology*
  • Interferon-gamma / pharmacology*
  • Monocytes / drug effects
  • Monocytes / immunology*
  • Monocytes / metabolism
  • Receptors, Very Late Antigen / immunology*
  • Recombinant Proteins
  • Tumor Necrosis Factor-alpha / pharmacology


  • CDw49d
  • Drug Combinations
  • HLA-DQ Antigens
  • Histocompatibility Antigens Class II
  • Integrin alpha Chains
  • Receptors, Very Late Antigen
  • Recombinant Proteins
  • Tumor Necrosis Factor-alpha
  • Intercellular Adhesion Molecule-1
  • Interferon-beta
  • Interferon-gamma