Stimulation by epigallocatechin gallate of interleukin-1 production by human peripheral blood mononuclear cells

Anticancer Res. 1995 May-Jun;15(3):971-4.


(-) Epigallocatechin gallate (EGCg) potently stimulated the production of interleukin-1 (IL-1) and tumor necrosis factor by human peripheral blood mononuclear cells. The intracellular amounts of IL-1 beta and especially IL-1 alpha induced by EGCg, were significantly higher than their extracellular counterparts. ECCg stimulated the production of adherent cells, with IL-1 producing capacity (per cell basis) that was significantly higher than nonadherent cells. Although IL-1 alpha mRNA synthesis (assessed by Reverse Transcriptase-Polymerase Chain Reaction) was slightly enhanced, IL-1 beta mRNA synthesis was not significantly enhanced by EGCg treatment. Lipopolysaccharide (LPS) also stimulated the production of IL-1 alpha and IL-1 beta production, but failed to induce the adherent cells. These data suggest that EGCg and LPS stimulate mononuclear cells by different mechanisms.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antineoplastic Agents / pharmacology*
  • Base Sequence
  • Catechin / analogs & derivatives*
  • Catechin / pharmacology
  • Cells, Cultured
  • DNA Primers
  • Humans
  • Interleukin-1 / biosynthesis*
  • Interleukin-1 / blood
  • Kinetics
  • Lymphocytes / drug effects
  • Lymphocytes / immunology*
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • RNA, Messenger / analysis
  • RNA, Messenger / biosynthesis
  • Time Factors
  • Tumor Necrosis Factor-alpha / biosynthesis


  • Antineoplastic Agents
  • DNA Primers
  • Interleukin-1
  • RNA, Messenger
  • Tumor Necrosis Factor-alpha
  • Catechin
  • epigallocatechin gallate