Endogenous interleukin 10 prevents apoptosis in macrophages during Salmonella infection

Biochem Biophys Res Commun. 1995 Aug 15;213(2):600-7. doi: 10.1006/bbrc.1995.2174.


To elucidate the biological roles of endogenous interleukin 10 (IL-10) in macrophage responses during bacterial infection, we examined in vitro effects of neutralizing IL-10 by anti-IL-10 monoclonal antibodies (mAb) on apoptosis of the peritoneal macrophages following Salmonella choleraesuis infection. Marked increments of TNF-alpha production were observed in the culture supernatant later than 6 h after in vitro culture with anti-IL-10 mAb. These macrophages succumbed to apoptosis at this stage accompanied by marked increment of IL-1 release, despite the expression of higher amount of endogenous heat shock protein 70, an inhibitor of TNF-alpha-mediated apoptosis. These results suggest that endogenous IL-10 plays an essential role in protection of Salmonella-infected macrophages from autocrine suicide caused by excessive production of TNF-alpha after killing of Salmonella.

MeSH terms

  • Animals
  • Antibodies, Monoclonal / pharmacology
  • Apoptosis*
  • Female
  • HSP70 Heat-Shock Proteins / biosynthesis
  • Interleukin-10 / antagonists & inhibitors
  • Interleukin-10 / physiology*
  • Macrophages, Peritoneal / microbiology
  • Macrophages, Peritoneal / physiology*
  • Mice
  • Mice, Inbred BALB C
  • Nitric Oxide / metabolism
  • Rats
  • Salmonella Infections, Animal / pathology*


  • Antibodies, Monoclonal
  • HSP70 Heat-Shock Proteins
  • Interleukin-10
  • Nitric Oxide