Tissue-regulated differentiation and maturation of a v-abl-immortalized mast cell-committed progenitor

Immunity. 1995 Aug;3(2):175-86. doi: 10.1016/1074-7613(95)90087-x.


An immature v-abl-transformed mast cell line (V3-MC) was derived from a mouse that developed systemic mastocytosis after transplantation of v-abl-infected bone marrow cells. V3-MCs injected intravenously into adult BALB/c mice infiltrated the liver, spleen, and intestine by day 6 and underwent progressive differentiation and maturation, eventually resembling indigenous mast cells. In terms of their protease content, the V3-MCs that localized in the liver and spleen differed from those in the intestine, and both differed from the cultured V3-MCs. The acquired expression of certain proteases and the loss of expression of other proteases in these tissue V3-MCs defines particular phenotypes and indicates that the differentiation and maturation of mast cell-committed progenitor cells are primarily regulated by factors in the different tissue microenvironments.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Base Sequence
  • Bone Marrow Cells
  • Cell Differentiation
  • DNA Primers / chemistry
  • Gene Expression
  • Genes, abl*
  • Immunohistochemistry
  • Mast Cells / cytology*
  • Mastocytosis / pathology*
  • Mice
  • Mice, Inbred BALB C
  • Molecular Sequence Data
  • Oncogene Proteins, Viral / physiology*
  • RNA, Messenger / genetics
  • Serine Endopeptidases / genetics
  • Serine Endopeptidases / metabolism
  • Tissue Distribution


  • DNA Primers
  • Oncogene Proteins, Viral
  • RNA, Messenger
  • Serine Endopeptidases