Isolation of mutant T lymphocytes with defects in capacitative calcium entry

Immunity. 1995 Aug;3(2):239-50. doi: 10.1016/1074-7613(95)90093-4.


Calcium and calcium-binding proteins play important roles in the signaling cascade leading from the initial engagement of TCRs on T cells to the fully activated state. To undertake a molecular dissection of this cascade, we first isolated a Jurkat T cell line derivative containing the NF-AT promoter element driving transcription of the diphtheria toxin A chain gene (dipA), resulting in rapid cell death. Selecting viable cells that fail to activate NF-AT-dependent transcription, we isolated two independent cell lines possessing defects in capacitative Ca2+ entry. NF-AT-dependent transcription can be restored in these cells by expression of a constitutively active calcineurin, but not overexpression of the Ca2+ regulatory protein CAML, which can normally replace the Ca2+ signal. The defect in these cell lines probably lies between CAML and calcineurin in the T cell activation cascade.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • Calcineurin
  • Calcium / physiology*
  • Calmodulin-Binding Proteins / physiology
  • Cell Compartmentation
  • Cell Line
  • Cell Nucleus / metabolism
  • DNA-Binding Proteins / metabolism
  • Gene Expression Regulation
  • Humans
  • Lymphocyte Activation
  • Molecular Sequence Data
  • Mutation
  • NFATC Transcription Factors
  • Nuclear Proteins*
  • Oligodeoxyribonucleotides / chemistry
  • Phosphoprotein Phosphatases / physiology
  • T-Lymphocytes / physiology*
  • Transcription Factors / metabolism


  • Calmodulin-Binding Proteins
  • DNA-Binding Proteins
  • NFATC Transcription Factors
  • Nuclear Proteins
  • Oligodeoxyribonucleotides
  • Transcription Factors
  • Calcineurin
  • Phosphoprotein Phosphatases
  • Calcium