Effects of transforming growth factor-beta on bone marrow macrophage Ia expression induced by cytokines

J Interferon Cytokine Res. 1995 May;15(5):485-91. doi: 10.1089/jir.1995.15.485.


The initiation of the immune response is regulated, in part, by the effect of cytokines on the level of expression of major histocompatibility complex (MHC) class II antigens on antigen-presenting cells (APC). The expression of class II antigens on B cell and macrophage APC is induced by IFN-gamma and IL-4, and GM-CSF induces class II expression on macrophages (M phi). Our results show that transforming growth factor-beta (TGF-beta) inhibits IL-4- and GM-CSF-induced Ia gene expression on bone marrow macrophages but enhances IFN-gamma-induced gene expression. Nuclear run-on experiments demonstrated that the inhibitory effects of TGF-beta on GM-CSF- and IL-4-induced Ia antigen expression were primarily posttranscriptional and augmentation of IFN-gamma by TGF-beta was largely transcriptionally regulated.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antigen Presentation / genetics
  • Antigen Presentation / immunology
  • Bone Marrow / immunology*
  • Bone Marrow Cells
  • Cell Line, Transformed
  • Cells, Cultured
  • Cytokines / immunology
  • Cytokines / pharmacology*
  • Gene Expression Regulation
  • Granulocyte-Macrophage Colony-Stimulating Factor / immunology
  • Granulocyte-Macrophage Colony-Stimulating Factor / pharmacology
  • Histocompatibility Antigens Class II / biosynthesis*
  • Histocompatibility Antigens Class II / genetics
  • Histocompatibility Antigens Class II / immunology
  • Interferon-gamma / immunology
  • Interferon-gamma / pharmacology
  • Interleukin-4 / immunology
  • Interleukin-4 / pharmacology
  • Macrophage Activation / genetics
  • Macrophage Activation / immunology
  • Macrophages / cytology
  • Macrophages / immunology*
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C3H
  • Mice, Inbred Strains
  • RNA Processing, Post-Transcriptional
  • RNA, Messenger / analysis
  • Recombinant Proteins / immunology
  • Recombinant Proteins / pharmacology
  • Transcription, Genetic
  • Transforming Growth Factor beta / pharmacology*


  • Cytokines
  • Histocompatibility Antigens Class II
  • RNA, Messenger
  • Recombinant Proteins
  • Transforming Growth Factor beta
  • Interleukin-4
  • Interferon-gamma
  • Granulocyte-Macrophage Colony-Stimulating Factor