Structural characterization of the pectic polysaccharide, rhamnogalacturonan-II

Carbohydr Res. 1995 Jul 10;271(1):15-29. doi: 10.1016/0008-6215(94)00002-w.

Abstract

An octasaccharide was released from sycamore cell wall rhamnogalacturonan-II (RG-II) by selective acid hydrolysis of the glycosidic linkages of apiosyl residues and purified to homogeneity by gel-permeation and high-performance anion-exchange chromatographies. The octasaccharide 1 contains a terminal nonreducing beta-L-arabinofuranosyl residue linked to position 2 of the alpha-L-rhamnopyranosyl residue of the aceric acid-containing heptasaccharide 2 that had been previously isolated from RG-II [M.W. Spellman et al. Carbohydr. Res., 122 (1983) 131-153]. Heptasaccharide 2 and octasaccharide 1 were found to be mono- or di-O-acetylated. The O-acetyl groups were located, by ESMSMS, on the terminal nonreducing 2-O-methyl-alpha-L-fucosyl residue and/or on the 2-linked beta-L-aceryl acid residue. Octasaccharide 1 and heptasaccharide 2 have the following structures: [structure: see text]

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Borohydrides
  • Carbohydrate Conformation
  • Carbohydrate Sequence
  • Chromatography, Gel
  • Chromatography, Ion Exchange
  • Hydrolysis
  • Mass Spectrometry
  • Molecular Sequence Data
  • Oligosaccharides / chemistry
  • Oligosaccharides / isolation & purification
  • Pectins / chemistry*
  • Sugar Alcohols / analysis

Substances

  • Borohydrides
  • Oligosaccharides
  • Sugar Alcohols
  • rhamnogalacturonan II
  • sodium borohydride
  • Pectins