Repression of transcriptional activity by heterologous KRAB domains present in zinc finger proteins

FEBS Lett. 1995 Aug 7;369(2-3):153-7. doi: 10.1016/0014-5793(95)00728-r.


We report the characterization of three novel members of the KRAB-domain containing C2-H2 zinc finger family (ZNF133, 136 and 140). KRAB (Krüppel-associated box) is an evolutionarily conserved protein domain found N-terminally with respect to the zinc finger repeats that encodes the DNA binding domain. ZNF133 and ZNF140 have both the KRAB A- and KRAB B-boxes present at their N-terminus, whereas ZNF136 contains only the KRAB A-box. We have previously demonstrated that the KRAB domains derived from ZNF133 and ZNF140 are potent transcriptional repression domains [Margolin et al. (1994) Proc. Natl. Acad. Sci. USA 91, 4509-4513]. The KRAB domain from ZNF136, containing only subdomain A, is a considerable weaker suppression domain; however, when fused to the heterologous KRAB B subdomain of ZNF10 (KOX1) the two subdomains from a KRAB domain which induces repression as potently as previously reported KRAB domains.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cloning, Molecular
  • Conserved Sequence
  • DNA-Binding Proteins / genetics*
  • Humans
  • Isomerases / blood
  • Isomerases / chemistry
  • Isomerases / genetics*
  • Molecular Sequence Data
  • Protein Disulfide-Isomerases*
  • Recombinant Fusion Proteins / biosynthesis
  • Repressor Proteins / blood
  • Repressor Proteins / chemistry
  • Repressor Proteins / genetics*
  • Saccharomyces cerevisiae Proteins*
  • Sequence Analysis, DNA
  • Transcription, Genetic
  • Zinc Fingers*


  • DNA-Binding Proteins
  • Recombinant Fusion Proteins
  • Repressor Proteins
  • Saccharomyces cerevisiae Proteins
  • ZNF133 protein, human
  • ZNF136 protein, human
  • ZNF140 protein, human
  • MPD1 protein, S cerevisiae
  • Isomerases
  • Protein Disulfide-Isomerases

Associated data

  • GENBANK/U09366
  • GENBANK/U09367
  • GENBANK/U09368