Molecular cloning and sequence analysis of human preprocathepsin C

FEBS Lett. 1995 Aug 7;369(2-3):326-30. doi: 10.1016/0014-5793(95)00777-7.


A cDNA clone (C1) coding for human preprocathepsin C was isolated from a human ileum cDNA library using a rat kidney-derived RT-PCR probe and its complete nucleotide sequence determined. The full-length 1857 bp sequence codes for a protein of 463 amino acid residues with a calculated molecular mass of 51848 Da. Comparison of the deduced amino acid sequence with that of rat preprocathepsin C indicates an 87.5% identity. A multiple alignment of the deduced cathepsin C sequence of 233 residues which, by analogy to other cystein proteinases, corresponds to the mature protein, confirms that human cathepsin C belongs to the papain superfamily.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cathepsins / chemistry
  • Cathepsins / genetics*
  • Cloning, Molecular
  • DNA, Complementary / genetics
  • Enzyme Precursors / chemistry
  • Enzyme Precursors / genetics*
  • Gene Dosage
  • Genes / genetics*
  • Humans
  • Molecular Sequence Data
  • Papain / genetics
  • Polymerase Chain Reaction / methods
  • Rats
  • Sequence Alignment
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid


  • DNA, Complementary
  • Enzyme Precursors
  • Cathepsins
  • preprocathepsin C
  • Papain

Associated data

  • GENBANK/X87212