Molecular cloning of NKB1. A natural killer cell receptor for HLA-B allotypes

J Immunol. 1995 Sep 1;155(5):2306-10.


The expression of certain MHC class I allotypes by potential target cells can inhibit NK cell-mediated cytotoxicity. We recently identified the NKB1 surface Ag, expressed on T and NK cell subsets, as a putative inhibitory receptor for HLA-B class I molecules possessing the Bw4 serologic epitope. NKB1 is a 70-kDa glycoprotein that after deglycosylation migrates as a 50-kDa protein as determined by SDS-PAGE. A cDNA encoding the NKB1 receptor was cloned from a NKB1+T cell cDNA library by expression in COS-7 cells using the anti-NKB1 mAb DX9. NKB1 is a member of the lg superfamily containing three lg-like domains in the extracellular region and is related to the recently identified family (p58/NKAT) of human NK and T cell surface molecules that appear to function as inhibitory receptors for HLA class I.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Blotting, Southern
  • Chromosome Mapping
  • Clone Cells
  • Cloning, Molecular*
  • DNA, Complementary / analysis*
  • Gene Expression
  • HLA-B Antigens*
  • Humans
  • Killer Cells, Natural / immunology
  • Molecular Sequence Data
  • RNA, Messenger / genetics
  • Receptors, Immunologic / genetics*
  • Receptors, KIR
  • Receptors, KIR2DL3
  • Receptors, KIR3DL1
  • Receptors, KIR3DS1
  • T-Lymphocytes / immunology


  • DNA, Complementary
  • HLA-B Antigens
  • KIR2DL3 protein, human
  • RNA, Messenger
  • Receptors, Immunologic
  • Receptors, KIR
  • Receptors, KIR2DL3
  • Receptors, KIR3DL1
  • Receptors, KIR3DS1

Associated data

  • GENBANK/U31416
  • GENBANK/U33328