Glycosylphosphatidylinositol-phospholipase D: A Tool for Glycosylphosphatidylinositol Structural Analysis

Methods Enzymol. 1995;250:630-40. doi: 10.1016/0076-6879(95)50101-0.

Abstract

Cleavage by the GPI-PLD provides definitive evidence of a minimal GPI structure: glucosamine-phosphatidylinositol. Unlike the case for PI-PLC, cleavage by the GPI-PLD is unaffected by acylation of the inositol ring. Thus the GPI-PLD provides an excellent simple enzymatic tool for analyzing the basic core structure of GPI anchors.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acylation
  • Animals
  • Carbohydrate Conformation
  • Carbohydrate Sequence
  • Cations, Divalent / pharmacology
  • Cattle
  • Chromatography, Affinity / methods
  • Chromatography, Gel / methods
  • Chromatography, Ion Exchange / methods
  • Cloning, Molecular / methods
  • DNA, Complementary
  • Detergents / pharmacology
  • Electrophoresis, Polyacrylamide Gel / methods
  • Escherichia coli
  • Gene Expression
  • Glucosamine / analysis
  • Glycosylphosphatidylinositols / chemistry*
  • Humans
  • Kinetics
  • Mammals
  • Molecular Conformation
  • Molecular Sequence Data
  • Molecular Weight
  • Phospholipase D / blood*
  • Phospholipase D / chemistry
  • Phospholipase D / isolation & purification*
  • Phosphorylation
  • Recombinant Proteins / chemistry
  • Substrate Specificity
  • Ultracentrifugation / methods

Substances

  • Cations, Divalent
  • DNA, Complementary
  • Detergents
  • Glycosylphosphatidylinositols
  • Recombinant Proteins
  • Phospholipase D
  • glycoprotein phospholipase D
  • Glucosamine