Immunochemical detection methods for Salmonella spp., Escherichia coli O157:H7, and Listeria monocytogenes in foods

Rev Environ Contam Toxicol. 1995:142:1-12. doi: 10.1007/978-1-4612-4252-9_1.

Abstract

Immunochemical assays should prove to be a significant improvement over standard culture methods for the detection of foodborne pathogens. These techniques take advantage of the specificity and sensitivity of the antibody/antigen reaction for analyte detection. ELISA is the most useful form of immunochemical method for the detection of foodborne pathogens in the food-processing setting, based on their simplicity and ability to analyze large numbers of samples at a time. Concerns with immunochemical techniques include problems with cross-reactivity and difficulties obtaining species specific assays. Also, most immunochemical methods continue to require an enrichment technique; however, subsequent identification is quite rapid when compared with standard culture methods, therefore allowing for the quicker release of negative products.

Publication types

  • Comparative Study
  • Review

MeSH terms

  • Culture Media
  • Escherichia coli / immunology
  • Escherichia coli / isolation & purification*
  • Food Microbiology*
  • Immunoassay
  • Listeria monocytogenes / immunology
  • Listeria monocytogenes / isolation & purification*
  • Salmonella / immunology
  • Salmonella / isolation & purification*

Substances

  • Culture Media