Detection of anti-bovine beta 2-glycoprotein I antibody in sera from patients with antiphospholipid syndrome

Rheumatol Int. 1995;15(1):1-8. doi: 10.1007/BF00286762.


We studied and characterized anti-bovine beta 2-glycoprotein I antibodies (aB beta 2-GPI) in sera from patients with antiphospholipid syndrome (APS) by ELISA. Bovine beta 2-glycoprotein I (beta 2-GPI) was purified by heparin affinity and DEAE ion-exchange chromatography, and identified on immunoblots using a monoclonal antibody against human beta 2-GPI and by amino acid sequence analysis. aB beta 2-GPI levels in the sera from 36 APS patients were measured by ELISA using purified bovine beta 2-GPI as an antigen. The mean +/- standard deviation level of aB beta 2-GPI was 17.4 +/- 22.0 units in the 58% of APS patients who were positive. There was a significant correlation (P = 0.003) between aB beta 2-GPI and anticardiolipin antibody (aCL) levels. aB beta 2-GPI from the sera of patients with APS was inhibited by bovine beta 2-GPI itself. Purified IgG from the sera of patients with APS showed that bovine beta 2-GPI was capable of acting as a cofactor for aCL. Purified bovine beta 2-GPI was useful antigen for conventional ELISA. aB beta 2-GPI may contribute to the further development of aCL analysis and to the understanding of the pathogenesis of APS.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Antibodies / blood*
  • Antibodies, Anticardiolipin / blood
  • Antibodies, Antiphospholipid / blood
  • Antibodies, Monoclonal
  • Antiphospholipid Syndrome / immunology*
  • Chromatography, Ion Exchange
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Glycoproteins / immunology*
  • Glycoproteins / isolation & purification
  • Humans
  • Immunoblotting
  • Immunoglobulin G / metabolism
  • Male
  • beta 2-Glycoprotein I


  • Antibodies
  • Antibodies, Anticardiolipin
  • Antibodies, Antiphospholipid
  • Antibodies, Monoclonal
  • Glycoproteins
  • Immunoglobulin G
  • beta 2-Glycoprotein I