Quantification of alpha 1-adrenoceptor subtypes in human tissues by competitive RT-PCR analysis

Biochem Biophys Res Commun. 1995 Aug 24;213(3):935-43. doi: 10.1006/bbrc.1995.2219.


Alpha 1-Adrenoceptors are a heterogeneous subfamily of receptors comprising at least three pharmacologically- and structurally- distinct subtypes named alpha 1a-, alpha 1b- and alpha 1d-adrenoceptors. A competitive reverse transcription-polymerase chain reaction methodology using subtype selective competitor RNAs as reference internal standards was adopted to determine the absolute amounts of each of the three cloned to date alpha 1-adrenoceptor subtype encoding mRNAs in selected human tissues. Our data demonstrated that each of these alpha 1-adrenoceptor subtypes exhibits a distinctive tissular expression pattern.

MeSH terms

  • Base Sequence
  • DNA Primers
  • Humans
  • Molecular Sequence Data
  • Polymerase Chain Reaction / methods
  • Receptors, Adrenergic, alpha-1 / classification
  • Receptors, Adrenergic, alpha-1 / metabolism*
  • Templates, Genetic
  • Transcription, Genetic


  • DNA Primers
  • Receptors, Adrenergic, alpha-1