Differential constitutive expression of interferon genes in early mouse embryos

Mol Reprod Dev. 1995 Jun;41(2):157-66. doi: 10.1002/mrd.1080410206.

Abstract

Recent evidence suggests that several processes during mammalian embryogenesis may be regulated by IFNs or IFN-like molecules. With the use of MAPPing, the simultaneous presence of transcripts homologous to IFN-alpha, IFN-beta, IRF-1, and IRF-2 was examined in mouse embryos and in embryonal carcinoma (EC) P19 cells, which are equivalent to epiblast cells of the early postimplantation blastocysts. Transcripts for IFN-alpha, but not for IFN-beta, were detected as maternal transcripts in the ovulated oocyte and persisted over early embryogenesis. IRF-1 transcripts appeared only after the first cell cleavage in the two-cell stage embryo. IRF-2 transcripts were analyzed only in EC P19 cells and were found in both undifferentiated (D-) and differentiated (D+) cells. The IFN-alpha transcripts present in (D-) P19 cells were cloned and the partial cDNA sequences determined. Mu IFN-alpha A and a new Mu IFN-alpha species (Mu IFN-alpha 12) were isolated from (D-) P19 cells. The presence of constitutive IFN-alpha transcripts in early mouse embryos suggests a role for these molecules during embryogenesis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Cloning, Molecular
  • Embryo, Mammalian / metabolism*
  • Embryonic and Fetal Development / genetics*
  • Embryonic and Fetal Development / physiology
  • Female
  • Gene Expression Regulation, Developmental*
  • Interferon-alpha / biosynthesis*
  • Interferon-alpha / genetics
  • Interferon-beta / biosynthesis*
  • Interferon-beta / genetics
  • Mice
  • Molecular Sequence Data
  • Pregnancy
  • Sequence Alignment
  • Transcription Factors / biosynthesis
  • Transcription Factors / genetics
  • Tumor Cells, Cultured

Substances

  • Interferon-alpha
  • Transcription Factors
  • Interferon-beta