Application of a bedside whole blood D-dimer assay in the diagnosis of deep vein thrombosis

Blood Coagul Fibrinolysis. 1995 May;6(3):219-22. doi: 10.1097/00001721-199505000-00004.


Cross-linked fibrin degradation products have been used to detect venous thrombosis. While the sensitivity of plasma D-dimer measured by ELISA in the diagnosis of deep vein thrombosis (DVT) is high, the utility of ELISA methods is limited in a clinical setting. This study analysed the diagnostic value of a rapid D-dimer assay performed on whole blood samples (SimpliRed D-dimer) compared with latex and ELISA in 86 patients suspected of having DVT. SimpliRed D-dimer was positive in 47/50 of patients with DVT established by Doppler ultrasound (DU; sensitivity 94%). SimpliRed D-dimer was positive in 35/37 of patients with proximal DVT, nine out of nine with popliteal DVT and three out of four with superficial thrombophlebitis. The specificity of SimpliRed D-dimer in the diagnosis of DVT was 61%. The sensitivity of the SimpliRed D-dimer assay was at least comparable with the ELISA (87%) and superior to the latex assay (80%). The positive predictive value (77%), the negative predictive value (88%) and the overall accuracy (80%) of the SimpliRed assay were better than the ELISA and latex methods. It is concluded that SimpliRed D-dimer is a rapid useful assay for screening of patients suspected of having deep vein thrombosis.

Publication types

  • Comparative Study

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Enzyme-Linked Immunosorbent Assay
  • Evaluation Studies as Topic
  • Fibrin Fibrinogen Degradation Products / analysis*
  • Fibrinolysis
  • Humans
  • Latex Fixation Tests
  • Middle Aged
  • Predictive Value of Tests
  • Reagent Kits, Diagnostic*
  • Sensitivity and Specificity
  • Thrombophlebitis / blood
  • Thrombophlebitis / diagnosis*
  • Thrombophlebitis / diagnostic imaging
  • Ultrasonography


  • Fibrin Fibrinogen Degradation Products
  • Reagent Kits, Diagnostic
  • fibrin fragment D