pBINPLUS: an improved plant transformation vector based on pBIN19

Transgenic Res. 1995 Jul;4(4):288-90. doi: 10.1007/BF01969123.


We describe the construction of a new plant transformation vector, pBINPLUS, based on the popular pBIN19 vector. Improvements over pBIN19 include location of the selectable marker gene at the left T-DNA border, a higher copy number in E. coli, and two rare restriction sites around the multiple cloning site for easier cloning and analysis of T-DNA insertions in plant genomes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Agrobacterium tumefaciens / genetics*
  • Amino Acid Oxidoreductases / genetics
  • Base Sequence
  • Cloning, Molecular / methods
  • DNA, Bacterial / genetics*
  • Genetic Vectors*
  • Kanamycin Kinase
  • Molecular Sequence Data
  • Phosphotransferases (Alcohol Group Acceptor) / genetics
  • Plants / genetics*
  • Plants, Genetically Modified
  • Plasmids
  • Replication Origin
  • Transformation, Genetic*


  • DNA, Bacterial
  • Amino Acid Oxidoreductases
  • nopaline synthase
  • Phosphotransferases (Alcohol Group Acceptor)
  • Kanamycin Kinase