The prognostic factors in 54 patients with osteosarcoma were studied by analyses of simple factor, multiple linear regression and stepwise regression. The results showed that the significant factors affecting prognosis were tumor cells' DNA content, radiological grade and surgical stage, while tumors' site, maximal diameter proliferating activity indicated by S-phase cell percentage, proliferating index and AgNOR counts had limited influence on the patients' outcome. Besides, patients' sex, age, duration of the symptom, tumors' radiological classification and pathological type bore no relation to survival rate.