Calcitonin gene-related peptide modulates acid-mediated regulation of somatostatin and gastrin release from rat antrum

Gastroenterology. 1995 Sep;109(3):701-6. doi: 10.1016/0016-5085(95)90376-3.


Background & aims: Acid has been shown to stimulate calcitonin gene-related peptide (CGRP) release from peripheral sensory afferent nerve endings in the stomach. The aim of this study was to determine whether endogenous CGRP was involved, by a neurocrine mechanism, in acid-mediated stimulation of somatostatin and inhibition of gastrin release.

Methods: A two-compartment sleeve of antral mucosal/submucosal tissue was perfused to determine sensory nerve and endocrine cell responses to luminal acid. CGRP receptor antagonist, CGRP8-37, was used to inhibit the actions of endogenously released CGRP.

Results: Perfusion of the antral sleeve lumen with media of increasing hydrogen ion concentration caused pH-dependent increases in CGRP and somatostatin release and decrease in gastrin release. CGRP8-37 inhibited significantly basal somatostatin (-36%) and stimulated basal gastrin (+65%) release (P < 0.02). Furthermore, CGRP8-37 administration prevented luminal acid-mediated inhibition of gastrin release and stimulation of somatostatin release. These results indicate that CGRP8-37 prevented acid-mediated feedback inhibition of gastrin release and acid-induced feedforward somatostatin release.

Conclusion: These results suggest that CGRP plays an important role in the response of antral D and G cells to luminal acid and that local effector action of endogenous CGRP participates in regulation of antral regulatory peptide secretion.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Analysis of Variance
  • Animals
  • Calcitonin Gene-Related Peptide / antagonists & inhibitors
  • Calcitonin Gene-Related Peptide / pharmacology
  • Calcitonin Gene-Related Peptide / physiology*
  • Gastric Acid / physiology*
  • Gastric Mucosa / drug effects
  • Gastric Mucosa / metabolism
  • Gastrins / metabolism*
  • Male
  • Peptide Fragments / pharmacology
  • Pyloric Antrum / drug effects
  • Pyloric Antrum / metabolism*
  • Radioimmunoassay
  • Rats
  • Rats, Sprague-Dawley
  • Somatostatin / metabolism*


  • Gastrins
  • Peptide Fragments
  • calcitonin gene-related peptide (8-37)
  • Somatostatin
  • Calcitonin Gene-Related Peptide