A three-dimensional model of the Rev-binding element of HIV-1 derived from analyses of aptamers

Nat Struct Biol. 1994 May;1(5):293-300. doi: 10.1038/nsb0594-293.

Abstract

Coordinated variations in the sequence of the Rev-binding element of HIV-1, identified by in vitro genetic selections, have been used as distance and conformational constraints for molecular modelling. Three-dimensional models of the wild-type Rev-binding element and several, evolved RNA ligands (aptamers) have been constructed. These models demonstrate that non-Watson-Crick pairings open the major groove allowing access of an alpha-helical peptide from Rev, and explain why some selected RNA sequences can bind Rev more tightly than others.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Composition
  • Base Sequence
  • Binding Sites
  • Computer Simulation*
  • Gene Products, rev / chemistry
  • Gene Products, rev / metabolism*
  • HIV-1 / metabolism*
  • Ligands
  • Models, Molecular*
  • Molecular Sequence Data
  • Nucleic Acid Conformation*
  • Protein Binding
  • Protein Conformation
  • RNA, Viral / chemistry*
  • RNA, Viral / metabolism*
  • rev Gene Products, Human Immunodeficiency Virus

Substances

  • Gene Products, rev
  • Ligands
  • RNA, Viral
  • rev Gene Products, Human Immunodeficiency Virus