Human papillomavirus-16 and -18 replication in esophagus squamous cancer cell lines does not require heterologous E1 and E2 proteins

J Med Virol. 1995 Apr;45(4):435-8. doi: 10.1002/jmv.1890450414.


Human papillomaviruses (HPVs) are double-stranded DNA viruses that replicate in the nuclei of squamous epithelial cells. HPVs can be classified into high-risk (e.g., types 16, 18, 31, and 33) or low-risk (e.g., types 6, 11, and 30), depending on their association with benign or malignant tumors. We recently described the association of HPV-16 and -18 with esophagus squamous cell cancer. HPV replication was studied in representative cell lines derived from esophagus cancers. HPV-16 and -18 genomes were independently transiently transfected into HCE-4 and HCE-7 cell lines with and without E1 and E2 genes under heterologous promoters. Southern blot analysis demonstrated that these cell lines support viral replication. However, heterologous E1 and E2 are not required for HPV replication. These findings suggest that specific host nuclear factors in esophageal squamous epithelial cells may support HPV replication.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blotting, Southern
  • Carcinoma, Squamous Cell / virology*
  • DNA, Viral / analysis
  • DNA-Binding Proteins*
  • Esophageal Neoplasms / virology*
  • Genetic Vectors
  • Genome, Viral
  • Humans
  • Oncogene Proteins, Fusion / genetics
  • Oncogene Proteins, Viral / genetics*
  • Papillomaviridae / classification
  • Papillomaviridae / genetics*
  • Papillomaviridae / isolation & purification
  • Promoter Regions, Genetic
  • Transfection
  • Tumor Cells, Cultured
  • Virus Replication


  • DNA, Viral
  • DNA-Binding Proteins
  • E1 protein, Human papillomavirus 16
  • E2 protein, Human papillomavirus type 16
  • Oncogene Proteins, Fusion
  • Oncogene Proteins, Viral
  • oncogene protein E1, Human papillomavirus type 18
  • oncogene protein E2, Human papillomavirus type 18