Expression of CD38 gene, but not of mitochondrial glycerol-3-phosphate dehydrogenase gene, is impaired in pancreatic islets of GK rats

Biochem Biophys Res Commun. 1995 Sep 5;214(1):239-46. doi: 10.1006/bbrc.1995.2280.


Goto-Kakizaki (GK) rat, a rodent model of spontaneously occurring non-insulin dependent diabetes mellitus (NIDDM), exhibits impaired glucose-stimulated insulin secretion. To explore the background of the beta-cell dysfunction in NIDDM, we investigated whether and how the expression pattern of factors that would potentially be involved in the glucose-stimulated insulin secretion machinery is changed in GK rats. Using quantitative reverse transcription-PCR (RT-PCR) method, we found that the gene expression of CD38, a type 2 membrane protein which has ADP-ribosyl cyclase activity, is reduced by approximately 50% in islets of GK rats. Despite previous studies showing reduction in the FAD-linked mitochondrial glycerol-3-phosphate dehydrogenase (mGPDH) activity in GK rats, the mGPDH mRNA amounts were equal to those in the control Wistar rats, suggesting a difference that arose post-transcriptionally. These observations support the idea that multiple defects of the glucose-responsive insulin secreting machinery are involved in the development of diabetes in GK rats.

MeSH terms

  • ADP-ribosyl Cyclase
  • ADP-ribosyl Cyclase 1
  • Animals
  • Antigens, CD / genetics*
  • Antigens, Differentiation / genetics*
  • Diabetes Mellitus, Type 2 / enzymology
  • Diabetes Mellitus, Type 2 / genetics*
  • Disease Models, Animal
  • Gene Expression Regulation*
  • Glucose / pharmacology
  • Glycerolphosphate Dehydrogenase / genetics*
  • Insulin / metabolism
  • Insulin Secretion
  • Islets of Langerhans / drug effects
  • Islets of Langerhans / metabolism*
  • Male
  • Membrane Glycoproteins
  • N-Glycosyl Hydrolases / genetics*
  • Polymerase Chain Reaction
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Rats
  • Rats, Wistar
  • Species Specificity


  • Antigens, CD
  • Antigens, Differentiation
  • Insulin
  • Membrane Glycoproteins
  • RNA, Messenger
  • Glycerolphosphate Dehydrogenase
  • N-Glycosyl Hydrolases
  • ADP-ribosyl Cyclase
  • Cd38 protein, rat
  • ADP-ribosyl Cyclase 1
  • Glucose