An inhibitory role for phosphatidylinositol 3-kinase in insulin secretion from pancreatic B cell line MIN6

Biochem Biophys Res Commun. 1995 Sep 5;214(1):51-9. doi: 10.1006/bbrc.1995.2255.


Phosphatidylinositol 3-kinase (PI3-kinase) has been implicated in the regulation of vesicular transport. We examined the roles of PI3-kinase in the glucose-induced insulin secretion from the pancreatic beta cell line MIN6 by using wortmannin, a potent inhibitor of PI3-kinase. Low concentrations of wortmannin markedly potentiated the glucose-induced insulin secretion. This effect was probably mediated by PI3-kinase inhibition. Furthermore, wortmannin completely canceled the suppressive effect of insulin-like growth factor-I on insulin secretion from MIN6 cells. On the basis of these results, we discuss a possible role of PI3-kinase in the negative feed-back regulation of insulin secretion.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Androstadienes / metabolism
  • Androstadienes / pharmacology
  • Cell Line
  • Insulin / metabolism*
  • Insulin Secretion
  • Insulin-Like Growth Factor I / pharmacology
  • Islets of Langerhans / enzymology
  • Islets of Langerhans / metabolism*
  • Phosphatidylinositol 3-Kinases
  • Phosphotransferases (Alcohol Group Acceptor) / antagonists & inhibitors
  • Phosphotransferases (Alcohol Group Acceptor) / metabolism*
  • Protein Binding
  • Proteins / metabolism
  • Somatostatin / pharmacology
  • Wortmannin


  • Androstadienes
  • Insulin
  • Proteins
  • Somatostatin
  • Insulin-Like Growth Factor I
  • Phosphatidylinositol 3-Kinases
  • Phosphotransferases (Alcohol Group Acceptor)
  • Wortmannin