A contravention of Ohno's law in mice

Nat Genet. 1995 Aug;10(4):472-6. doi: 10.1038/ng0895-472.


The chloride channel gene, CLCN4, has been previously mapped to the X chromosome in humans. We isolated a cDNA clone for mouse Clcn4 and used this to map the gene in an interspecific backcross. This revealed the surprising finding that the gene maps to the X chromosome in Mus spretus but to chromosome 7 in C57BL/6 mice. This is the first example of a gene that contravenes Ohno's law--it is a gene unique to the X chromosome in one eutherian species but autosomal in another. The consequence of this chromosomal rearrangement is that the gene is lost by mendelian segregation in a subset of the male progeny of a (C57BL/6 x Mus spretus) x Mus spretus backcross.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Chloride Channels / genetics*
  • Chromosome Mapping*
  • Cloning, Molecular
  • Crosses, Genetic
  • DNA
  • Dosage Compensation, Genetic
  • Female
  • Humans
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Models, Genetic
  • Molecular Sequence Data
  • Muridae
  • Rats
  • X Chromosome*


  • CLC-5 chloride channel
  • CLCN4 protein, human
  • Chloride Channels
  • DNA