Biochemical and molecular characterization of a novel starch synthase from potato tubers

Plant J. 1995 Aug;8(2):283-94. doi: 10.1046/j.1365-313x.1995.08020283.x.

Abstract

An isoform of starch synthase from potato tubers which is present both in the stroma of the plastid and tightly bound to starch granules has been identified biochemically and a cDNA has been isolated. The protein encoded by the cDNA is 79.9 kDa and has a putative transit peptide and a distinct N-terminal domain which is predicted to be highly flexible. It is similar in both amino acid sequence and predicted structure to the granule-bound starch synthase II (GBSSII) of pea embryos. When expressed in Escherichia coli, the mature protein has starch synthase activity. The importance of the isoform has been assessed by biochemical measurements and antisense transformation experiments in which the amount of the isoform in the tuber is severely and specifically reduced. Both approaches indicate that the isoform contributes a maximum of 15% of the total starch synthase activity of the tuber. It is suggested that this isoform and the GBSSII of pea embryos represent a widely distributed class of isoforms of starch synthase. The contribution to total starch synthase activity of members of this class probably varies considerably from one type of storage organ to another.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Blotting, Southern
  • Cloning, Molecular
  • DNA, Complementary / genetics
  • Escherichia coli / genetics
  • Immunoblotting
  • Isoenzymes / biosynthesis
  • Isoenzymes / genetics
  • Isoenzymes / metabolism*
  • Molecular Sequence Data
  • Organelles / enzymology
  • Plant Stems / enzymology*
  • Plant Stems / genetics
  • Precipitin Tests
  • RNA, Antisense
  • Recombinant Proteins / biosynthesis
  • Solanum tuberosum / enzymology*
  • Solanum tuberosum / genetics
  • Starch / biosynthesis*
  • Starch Synthase / biosynthesis
  • Starch Synthase / genetics
  • Starch Synthase / metabolism*
  • Subcellular Fractions / enzymology
  • Transformation, Genetic

Substances

  • DNA, Complementary
  • Isoenzymes
  • RNA, Antisense
  • Recombinant Proteins
  • Starch
  • Starch Synthase

Associated data

  • GENBANK/X87988