Enhancing effect of platelet-derived growth factors on migration of corneal endothelial cells

Cornea. 1995 Jul;14(4):365-71. doi: 10.1097/00003226-199507000-00004.


To investigate the role of platelet-derived growth factor (PDGF) in corneal wound healing, we examined the effect of human natural PDGF, recombinant PDGF-BB, and PDGF-AA on the migration of rabbit corneal endothelial cells. In a modified Boyden chamber in fetal bovine serum-free conditions, natural PDGF and PDGF-BB, at a concentration of 1-3 or 10 ng/ml, enhanced the migration of endothelial cells, whereas at a higher concentration (10 or 30 ng/ml), this enhanced migration was suppressed; the optimal concentration range for enhancing migration was 3-10 ng/ml. PDGF-AA did not enhance the migration. Natural PDGF and PDGF-BB activity was found for up to 6 h after the beginning of culture, and was completely blocked by anti-PDGF neutralizing antibodies. A checkerboard assay demonstrated that PDGF-BB had a chemotactic effect on the corneal endothelial cell migration. These results suggest that natural PDGF and PDGF-BB, but not PDGF-AA, are involved in corneal wound healing by stimulating the migration of corneal endothelial cells.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Antibodies / pharmacology
  • Becaplermin
  • Cells, Cultured
  • Chemotaxis / drug effects
  • Chemotaxis / physiology*
  • Culture Media, Serum-Free
  • Endothelium, Corneal / cytology*
  • Endothelium, Corneal / drug effects
  • Endothelium, Corneal / physiology
  • Platelet-Derived Growth Factor / immunology
  • Platelet-Derived Growth Factor / pharmacology*
  • Proto-Oncogene Proteins c-sis
  • Rabbits
  • Recombinant Proteins
  • Wound Healing / physiology


  • Antibodies
  • Culture Media, Serum-Free
  • Platelet-Derived Growth Factor
  • Proto-Oncogene Proteins c-sis
  • Recombinant Proteins
  • platelet-derived growth factor A
  • Becaplermin