Protein insertion into the endoplasmic reticulum of permeabilized cells

FEBS Lett. 1995 Sep 4;371(2):145-8. doi: 10.1016/0014-5793(95)00881-9.

Abstract

We have established efficient translocation of newly synthesized proteins into the endoplasmic reticulum of permeabilized Mel Juso cells. By site-specific photo-crosslinking we show that translocating polypeptide chains contact the same components of permeabilized cells ER as in dog pancreas rough microsomes. This cellular assay system has the potential to overcome the limitations of isolated microsomes in investigating the molecular environment of a newly synthesized protein after they have left the ER translocation site.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biological Transport
  • Cell Line
  • Cell Membrane Permeability*
  • Codon
  • Cross-Linking Reagents
  • Dogs
  • Endopeptidase K
  • Endoplasmic Reticulum / metabolism*
  • Endoplasmic Reticulum / ultrastructure
  • Glycosylation
  • Intracellular Membranes / metabolism
  • Light
  • Membrane Proteins / metabolism*
  • Octoxynol
  • Pancreas / ultrastructure
  • Protein Biosynthesis
  • RNA, Messenger / metabolism
  • Saponins / pharmacology
  • Serine Endopeptidases / pharmacology
  • Solubility

Substances

  • Codon
  • Cross-Linking Reagents
  • Membrane Proteins
  • RNA, Messenger
  • Saponins
  • Octoxynol
  • Serine Endopeptidases
  • Endopeptidase K