Participation of a Proton-Cotransporter, MCT1, in the Intestinal Transport of Monocarboxylic Acids

Biochem Biophys Res Commun. 1995 Sep 14;214(2):482-9. doi: 10.1006/bbrc.1995.2312.

Abstract

A molecular mechanism for the intestinal monocarboxylic acid transport was characterized by using a proton/monocarboxylate transporter, MCT1, in Chinese hamster ovary (CHO) cells, first found by Garcia et al. (Cell, 76, 865-873, 1994). Northern blotting analysis showed that MCT1-isomers exist in the rat and rabbit intestinal enterocytes and Caco-2 cells. The expression of [14C]lactic acid uptake by Xenopus laevis oocytes injected with rabbit intestinal mRNA was reduced by hybridizing the mRNA with a MCT1 cDNA of CHO cells before microinjection used as the antisense DNA. [14C]Lactic acid uptake by CHO cells was pH dependent, saturable, stereospecific, and reduced in the presence of acetic acid, benzoic acid, S- and R-ibuprofen, S- and R-mandelic acid, nicotinic acid, pravastatin, propionic acid and valproic acid. In addition, several monocarboxylic acids were transported in pH-dependent and saturable manners. These results suggest that the intestinal MCT1-related protein contributes to a carrier-mediated absorption for organic weak acid compounds.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biological Transport / drug effects
  • CHO Cells
  • Carboxylic Acids / metabolism*
  • Carrier Proteins / biosynthesis
  • Carrier Proteins / metabolism*
  • Cricetinae
  • Female
  • Hydrogen-Ion Concentration
  • Ibuprofen / pharmacology
  • Intestinal Mucosa / metabolism*
  • Lactates / metabolism
  • Mandelic Acids / pharmacology
  • Membrane Proteins / metabolism
  • Monocarboxylic Acid Transporters
  • Niacin / metabolism
  • Niacin / pharmacology
  • Oocytes / physiology
  • Pravastatin / pharmacology
  • Propionates / pharmacology
  • Protons
  • RNA, Complementary
  • RNA, Messenger / metabolism
  • Rabbits
  • Recombinant Proteins / metabolism
  • Stereoisomerism
  • Transcription, Genetic
  • Transfection
  • Valproic Acid / pharmacology
  • Xenopus laevis

Substances

  • Carboxylic Acids
  • Carrier Proteins
  • Lactates
  • Mandelic Acids
  • Membrane Proteins
  • Monocarboxylic Acid Transporters
  • Propionates
  • Protons
  • RNA, Complementary
  • RNA, Messenger
  • Recombinant Proteins
  • Niacin
  • Valproic Acid
  • propionic acid
  • Pravastatin
  • mandelic acid
  • Ibuprofen