Presence of centrin in the human parasite Giardia: a further indication of its ubiquity in eukaryotes

Biochem Biophys Res Commun. 1995 Sep 14;214(2):597-601. doi: 10.1006/bbrc.1995.2327.

Abstract

The presence and distribution of the cytoskeletal protein centrin in the lower eukaryote Giardia were determined using a monoclonal antibody to algal centrin. Immunofluorescence shows that centrin is a major component of rodlike structures associated with the flagella and strongly attached to the ventral disc and the 2 nuclei. Two groups of spots are also highlighted at the top of each nucleus where the basal bodies are situated. Western blot analysis revealed a single 23-kDa polypeptide. These findings support the ubiquity of centrin and its ancient evolutionary origin and also underline cytoskeleton complexity of Giardia, the reportedly most primitive eukaryotic organism known.

MeSH terms

  • Animals
  • Blotting, Western
  • Calcium-Binding Proteins / analysis*
  • Chromosomal Proteins, Non-Histone*
  • Contractile Proteins / analysis*
  • Cytoskeletal Proteins / analysis*
  • Cytoskeleton / chemistry
  • Cytoskeleton / ultrastructure*
  • Electrophoresis, Polyacrylamide Gel
  • Fluorescent Antibody Technique
  • Giardia lamblia / chemistry
  • Giardia lamblia / cytology*
  • Humans
  • Microscopy, Phase-Contrast
  • Molecular Weight
  • Protozoan Proteins / analysis*

Substances

  • Calcium-Binding Proteins
  • Chromosomal Proteins, Non-Histone
  • Contractile Proteins
  • Cytoskeletal Proteins
  • Protozoan Proteins
  • caltractin