Detection of Chlamydia trachomatis deoxyribonucleic acid in women with tubal infertility

Fertil Steril. 1993 Jan;59(1):45-50.


Objective: To determine the presence of Chlamydia trachomatis by in situ hybridization in fallopian tube tissues of women with distal tubal occlusion.

Patients: Subjects were selected from a Seattle-based study of infertility in women with distally occluded fallopian tubes undergoing reconstructive surgery. For comparison, six specimens were obtained from women undergoing surgery for noninfertility-related conditions who had normal appearing tubes.

Methods: Tissue specimens from 16 of these patients were selected for analysis by in situ hybridization and immunoperoxidase (IP) staining.

Results: C. trachomatis was detected in 9 of 16 women by either in situ hybridization or IP. Six of the nine were seropositive with titers ranging from 1:16 to 1:64. Tissue from 6 women with normal appearing fallopian tubes were negative by in situ hybridization.

Conclusions: The demonstration of C. trachomatis deoxyribonucleic acid and/or antigens in fallopian tube tissue from infertile women with distal tubal disease suggests that C. trachomatis can persist in these tissues in an uncultivable state.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Biopsy
  • Chlamydia trachomatis / genetics*
  • DNA, Bacterial / analysis*
  • Fallopian Tube Diseases / complications*
  • Fallopian Tube Diseases / pathology
  • Fallopian Tubes / chemistry
  • Fallopian Tubes / pathology
  • Female
  • Humans
  • Immunoenzyme Techniques
  • In Situ Hybridization
  • Infertility, Female / etiology*
  • Infertility, Female / genetics
  • Infertility, Female / microbiology
  • Staining and Labeling


  • DNA, Bacterial