LH-2: a LIM/homeodomain gene expressed in developing lymphocytes and neural cells

Proc Natl Acad Sci U S A. 1993 Jan 1;90(1):227-31. doi: 10.1073/pnas.90.1.227.

Abstract

A screen for early markers of B-lymphocyte differentiation has identified a homeobox gene, denoted LH-2, that has a pattern of expression distinct from that of other related genes. The LH-2 cDNA sequence encodes a polypeptide of 426 amino acids that contains a homeodomain and two repeats of a cysteine-rich domain referred to as a LIM domain. The homeodomain of the LH-2 protein is related to that of other LIM/homeodomain proteins, most strikingly with that of the Drosophila apterous protein. Expression of LH-2 was found in B- and T-lymphoid cell lines. Expression in B-cell lines was highest in lines that represent early stages of differentiation, whereas in T-cell lines there was no clear correlation with the stage of differentiation. In embryonic and adult tissues, the highest level of LH-2 expression was found in discrete regions of the developing central nervous system, primarily in diencephalic and telencephalic structures, and in a subset of lymphoid tissues. The expression pattern and structural characteristics of the LH-2 gene suggest that it encodes a transcriptional regulatory protein involved in the control of cell differentiation in developing lymphoid and neural cell types.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Aging / physiology
  • Amino Acid Sequence
  • Animals
  • B-Lymphocytes / physiology*
  • Base Sequence
  • Brain / physiology*
  • Cell Differentiation
  • Cell Line
  • Cloning, Molecular
  • Cysteine
  • DNA / genetics
  • DNA / isolation & purification
  • Embryo, Mammalian
  • Gene Expression
  • Genes, Homeobox*
  • Homeodomain Proteins*
  • In Situ Hybridization
  • LIM-Homeodomain Proteins
  • Mice
  • Molecular Sequence Data
  • Organ Specificity
  • RNA / genetics
  • RNA / isolation & purification
  • RNA, Messenger / analysis
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism*
  • Rats
  • Sequence Homology, Amino Acid
  • Transcription Factors / genetics*
  • Transcription, Genetic*

Substances

  • Homeodomain Proteins
  • LIM-Homeodomain Proteins
  • Lhx2 protein, mouse
  • Lhx2 protein, rat
  • RNA, Messenger
  • Transcription Factors
  • RNA
  • DNA
  • Cysteine

Associated data

  • GENBANK/L06804