Human CD40-ligand: molecular cloning, cellular distribution and regulation of expression by factors controlling IgE production

FEBS Lett. 1993 Jan 11;315(3):259-66. doi: 10.1016/0014-5793(93)81175-y.

Abstract

Here we report the cloning of the cDNA for human CD40-Ligand (CD40-L) from a CD4-positive T cell clone. The deduced amino acid sequence predicts a type II membrane protein of 261 amino acids. Northern blot and FACS analysis of PBMNC revealed that the human CD40-L can be detected on T cells and is absent from B cells and monocytes. The human CD40-L is expressed on both CD4- and CD8-positive T cells, (CD45R0+) and (CD45RA+) subsets. We observed that IL-4, an inducer of IgE production, upregulated CD40-L mRNA level while IFN gamma, an inhibitor of IgE synthesis, reduced the expression of CD40-L mRNA. These data suggest a the correlation between human CD40-L expression and IgE production.

MeSH terms

  • Amino Acid Sequence
  • Antigens, CD*
  • Antigens, Differentiation, B-Lymphocyte*
  • Base Sequence
  • Blotting, Northern
  • CD40 Antigens
  • CD40 Ligand
  • Cells, Cultured
  • Cloning, Molecular
  • Cytokines / physiology
  • DNA
  • Flow Cytometry
  • Gene Expression Regulation*
  • Humans
  • Immunoglobulin E / biosynthesis*
  • Ligands
  • Membrane Glycoproteins / genetics*
  • Molecular Sequence Data
  • Neutrophils / metabolism
  • RNA, Messenger / genetics
  • Sequence Homology, Amino Acid

Substances

  • Antigens, CD
  • Antigens, Differentiation, B-Lymphocyte
  • CD40 Antigens
  • Cytokines
  • Ligands
  • Membrane Glycoproteins
  • RNA, Messenger
  • CD40 Ligand
  • Immunoglobulin E
  • DNA

Associated data

  • GENBANK/L07414