Isolation and characterization of a rice cDNA similar to the bovine brain-specific 14-3-3 protein gene

Plant Mol Biol. 1993 Jan;21(1):191-4. doi: 10.1007/BF00039631.

Abstract

We isolated a rice cDNA clone which is similar to the bovine brain-specific 14-3-3 protein (an activator protein of tyrosine and tryptophan hydroxylase involved in the synthetic pathway of monoamine) gene. The deduced amino acid sequence of the cDNA indicated a surprising similarity to a potent inhibitor of Ca(2+)-phospholipid-dependent protein kinase C. DNA blot analysis indicated that this gene is located at more than a single locus in rice genome DNA. Expression of this gene is regulated by external stresses.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • 14-3-3 Proteins
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Northern
  • Blotting, Southern
  • Brain / physiology*
  • Cattle
  • Cloning, Molecular
  • DNA / genetics*
  • DNA / isolation & purification
  • Molecular Sequence Data
  • Nerve Tissue Proteins / genetics*
  • Oryza / genetics*
  • Plants / genetics
  • Protein Kinase C / antagonists & inhibitors
  • RNA / genetics
  • RNA / isolation & purification
  • Restriction Mapping
  • Sequence Homology, Amino Acid
  • Sheep
  • Tyrosine 3-Monooxygenase*

Substances

  • 14-3-3 Proteins
  • Nerve Tissue Proteins
  • RNA
  • DNA
  • Tyrosine 3-Monooxygenase
  • Protein Kinase C

Associated data

  • GENBANK/D10436