The F3/11 cell adhesion molecule mediates the repulsion of neurons by the extracellular matrix glycoprotein J1-160/180

Neuron. 1993 Jan;10(1):69-82. doi: 10.1016/0896-6273(93)90243-k.

Abstract

The oligodendrocyte-derived extracellular matrix protein J1-160/180 displays repellent substrate properties toward neurons. In a search for neuronal ligands mediating the response to J1-160/180, we have identified the F3/11 cell surface protein, a glyco-phosphatidylinositol-anchored member of the immunoglobulin superfamily. F3/11 mediates the initial recognition between a J1-160/180 substrate and cerebellar neurons or F3-transfected CHO cells. In cerebellar neurons, the F3/11-J1-160/180 interaction induces a repulsion consisting of the loss of substrate adhesion with time in culture and inhibition of neurite outgrowth. Antibody blocking experiments show that the avoidance response of neurites at J1-160/180 substrate borders is also mediated by F3/11. Active cell-cell and cell-substrate repulsion is considered a major mechanism governing the extent and directionality of axonal growth, but the ligand-receptor interactions involved have remained unknown. Our results show that F3/11 mediates the neuronal response to the repellent molecule J1-160/180 and may thus be involved in signal transduction leading to cell repulsion.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal
  • CHO Cells
  • Cell Adhesion
  • Cell Adhesion Molecules, Neuronal / genetics
  • Cell Adhesion Molecules, Neuronal / immunology
  • Cell Adhesion Molecules, Neuronal / metabolism
  • Cell Adhesion Molecules, Neuronal / physiology*
  • Cells, Cultured
  • Cerebellum / cytology
  • Contactins
  • Cricetinae
  • DNA / genetics
  • Extracellular Matrix Proteins / immunology
  • Extracellular Matrix Proteins / metabolism
  • Extracellular Matrix Proteins / physiology*
  • Laminin / pharmacology
  • Mice
  • Mice, Inbred ICR
  • Neural Cell Adhesion Molecules*
  • Neurites / physiology
  • Neurons / physiology*
  • Oligodendroglia / chemistry
  • Polylysine / pharmacology
  • Tenascin
  • Transfection

Substances

  • Antibodies, Monoclonal
  • Cell Adhesion Molecules, Neuronal
  • Contactins
  • Extracellular Matrix Proteins
  • Laminin
  • Neural Cell Adhesion Molecules
  • Tenascin
  • tenascin R
  • Polylysine
  • DNA

Grant support