Soluble stem cell factor in human serum

Blood. 1993 Feb 1;81(3):656-60.

Abstract

Stem cell factor (SCF) is a recently described factor active in the early stages of hematopoiesis. It can exist in membrane-bound form and in proteolytically released soluble form. The levels and nature of SCF in human serum are described. As determined by an enzyme-linked immunosorbent assay performed for 257 samples, SCF level in serum averaged 3.3 +/- 1.1 ng/mL. The serum SCF was partially purified by immunoaffinity chromatography and analyzed by glycosidase treatments in conjunction with sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting. The results show that the SCF has N-linked and O-linked carbohydrate and corresponds to the soluble form, at or about 165 amino acids in length. The findings suggest functional importance for soluble SCF in humans.

MeSH terms

  • Adult
  • Age Factors
  • Cells, Cultured
  • Chromatography, Affinity
  • Colony-Forming Units Assay
  • Dose-Response Relationship, Drug
  • Electrophoresis, Polyacrylamide Gel
  • Enzyme-Linked Immunosorbent Assay / methods
  • Erythropoietin / pharmacology
  • Female
  • Hematopoietic Cell Growth Factors / blood*
  • Hematopoietic Cell Growth Factors / isolation & purification
  • Hematopoietic Cell Growth Factors / pharmacology
  • Humans
  • Immunoglobulin G
  • Leukocytes / cytology
  • Leukocytes / drug effects
  • Male
  • Middle Aged
  • Recombinant Proteins / pharmacology
  • Reference Values
  • Sex Characteristics
  • Stem Cell Factor

Substances

  • Hematopoietic Cell Growth Factors
  • Immunoglobulin G
  • Recombinant Proteins
  • Stem Cell Factor
  • Erythropoietin