Interleukin-1-induced expression of nitric oxide synthase in insulin-producing cells is preceded by c-fos induction and depends on gene transcription and protein synthesis

FEBS Lett. 1993 Feb 8;317(1-2):62-6. doi: 10.1016/0014-5793(93)81492-i.

Abstract

The cytokine interleukin 1 beta (IL-1 beta) induces the expression of an isoform of nitric oxide synthase (NOS) in insulin-producing cells which is similar to that expressed in activated macrophages. In the present study we show that IL-1 beta-induced expression of NOS mRNA in these cells is preceded by expression of c-fos mRNA. Moreover, the stimulatory effects of recombinant IL-1 beta on NOS mRNA expression are prevented by co-incubation with an inhibitor of gene transcription (actinomycin D) or an inhibitor of protein synthesis (cycloheximide). These data suggest that IL-1 beta-induced NOS mRNA expression may be mediated by transcription of immediate early response genes, and that c-fos may be one of these genes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Oxidoreductases / genetics*
  • Amino Acid Oxidoreductases / metabolism
  • Animals
  • Cell Line
  • Cricetinae
  • Cycloheximide / pharmacology
  • Dactinomycin / pharmacology
  • Enzyme Activation
  • Genes, fos*
  • Humans
  • Insulin / biosynthesis
  • Interleukin-1 / pharmacology*
  • Nitric Oxide Synthase
  • Pancreas / cytology
  • Pancreas / metabolism
  • Protein Biosynthesis
  • RNA, Messenger / metabolism
  • Receptor, Insulin / biosynthesis
  • Receptor, Insulin / genetics
  • Transcription, Genetic*

Substances

  • Insulin
  • Interleukin-1
  • RNA, Messenger
  • Dactinomycin
  • Cycloheximide
  • Nitric Oxide Synthase
  • Amino Acid Oxidoreductases
  • Receptor, Insulin