Expression of transforming growth factor-alpha in mid-gestation human fetal lung

Am J Respir Cell Mol Biol. 1993 Mar;8(3):266-72. doi: 10.1165/ajrcmb/8.3.266.


Transforming growth factor-alpha (TGF-alpha), a member of the epidermal growth factor (EGF) family, is a potent mitogen for several cell types. To investigate the possible role of TGF-alpha in the development of midgestation human fetal lung, we studied its distribution with immunohistochemistry and determined levels of steady-state TGF-alpha mRNA by Northern analysis of cellular RNA isolates from lung. Lung was obtained from fetuses at 10 to 22 wk of gestation (n = 14) and immunostained for TGF-alpha. TGF-alpha was localized in epithelial cells at all gestational ages examined. Immunostaining was particularly prominent in bronchiolar epithelial cells. TGF-alpha immunoreactivity was also associated with arterial smooth muscle cells, as well as with nerves. Occasional chondrocytes were also associated with TGF-alpha immunoreactivity. Total cellular RNA was isolated from lung tissue obtained from additional fetuses at gestational ages 10 to 24 wk (n = 22). TGF-alpha mRNA was present in RNA extracts of all fetal lungs studied. We conclude that TGF-alpha is probably produced in human fetal lung during mid-gestation. The prominent immunostaining of bronchiolar epithelial cells for TGF-alpha is consistent with its playing a role in distal airway formation.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antibodies, Monoclonal
  • Blotting, Northern
  • Female
  • Fetus
  • Gestational Age
  • Humans
  • Immunohistochemistry
  • Lung / cytology
  • Lung / embryology*
  • Pregnancy
  • Pregnancy Trimester, First
  • Pregnancy Trimester, Second
  • RNA / isolation & purification
  • RNA Probes
  • RNA, Messenger / analysis
  • RNA, Messenger / metabolism*
  • Recombinant Proteins / analysis
  • Recombinant Proteins / immunology
  • Transforming Growth Factor alpha / analysis
  • Transforming Growth Factor alpha / biosynthesis*
  • Transforming Growth Factor alpha / genetics


  • Antibodies, Monoclonal
  • RNA Probes
  • RNA, Messenger
  • Recombinant Proteins
  • Transforming Growth Factor alpha
  • RNA