Liquid holding effects on methylmercury genotoxicity in human lymphocytes

Mutat Res. 1993 Apr;301(4):267-73. doi: 10.1016/0165-7992(93)90068-7.

Abstract

Methylmercury chloride (MMC) treatment of resting (G0) human lymphocytes resulted in the formation of chromosome and chromatid aberrations. This treatment also induced aneuploidy in second metaphases, suggesting that MMC produces stable damage involved in chromosome segregation errors. The storage of treated cells (liquid holding for 48 h before cell proliferation) did not result in an important recovery from induced cell toxicity or chromosome damage. Therefore, MMC seems to be an X-ray-like agent, able to produce long-lasting damages giving rise to both structural and numerical chromosome abnormalities.

MeSH terms

  • Aneuploidy
  • Chromosome Aberrations*
  • Cytological Techniques
  • DNA Damage
  • DNA Repair
  • Humans
  • Hypotonic Solutions
  • Lymphocytes / drug effects*
  • Male
  • Metaphase / drug effects
  • Methylmercury Compounds / toxicity*
  • Middle Aged
  • Mitosis / drug effects*
  • Mutagens / toxicity*
  • Resting Phase, Cell Cycle / drug effects
  • Sister Chromatid Exchange

Substances

  • Hypotonic Solutions
  • Methylmercury Compounds
  • Mutagens
  • methylmercuric chloride