Primary structure and functional expression of a mouse inward rectifier potassium channel

Nature. 1993 Mar 11;362(6416):127-33. doi: 10.1038/362127a0.

Abstract

A complementary DNA encoding an inward rectifier K+ channel (IRK1) was isolated from a mouse macrophage cell line by expression cloning. This channel conducts inward K+ current below the K+ equilibrium potential but passes little outward K+ current. The IRK1 channel contains only two putative transmembrane segments per subunit and corresponds to the inner core structure of voltage-gated K+ channels. The IRK1 channel and an ATP-regulated K+ channel show extensive sequence similarity and constitute a new superfamily.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cattle
  • Cell Line
  • Cloning, Molecular
  • DNA / genetics*
  • DNA / isolation & purification
  • Gene Library
  • Guinea Pigs
  • Macrophages / physiology*
  • Membrane Proteins / genetics*
  • Membrane Proteins / physiology
  • Mice
  • Models, Structural
  • Molecular Sequence Data
  • Multigene Family*
  • Oocytes / physiology
  • Poly A / genetics
  • Poly A / isolation & purification
  • Potassium Channels / genetics*
  • Potassium Channels / physiology
  • Potassium Channels, Inwardly Rectifying*
  • RNA / genetics
  • RNA / isolation & purification
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Rats
  • Recombinant Proteins / metabolism
  • Sequence Homology, Amino Acid
  • Xenopus

Substances

  • Membrane Proteins
  • Potassium Channels
  • Potassium Channels, Inwardly Rectifying
  • RNA, Messenger
  • Recombinant Proteins
  • Poly A
  • RNA
  • DNA