Affinity purification of antibodies specific for Asn-linked glycans containing alpha 1-->3 fucose or beta 1-->2 xylose

Anal Biochem. 1993 Feb 15;209(1):104-8. doi: 10.1006/abio.1993.1088.

Abstract

Antisera raised against the plant glycoproteins beta-fructosidase and horseradish peroxidase can be fractionated on an affinity column of honeybee venom phospholipase A2 to produce serum fractions that are specific for either the alpha 1-->3 fucose or beta 1-->2 xylose epitopes commonly found on the Asn-linked glycans of plant glycoproteins. This affinity purification strategy relies on the absence of beta 1-->2 xylose from the glycan of the venom protein. Such antibody preparations can be used for the detection of these sugar epitopes on glycoproteins.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Antibodies / immunology
  • Antibodies / isolation & purification*
  • Antibody Specificity
  • Asparagine / analysis*
  • Bee Venoms / enzymology
  • Carbohydrate Sequence
  • Chromatography, Affinity / methods
  • Enzymes, Immobilized
  • Epitopes / analysis
  • Fucose / analysis
  • Fucose / immunology*
  • Glycoproteins / immunology
  • Glycoside Hydrolases / immunology
  • Horseradish Peroxidase / immunology
  • Molecular Sequence Data
  • Phospholipases A
  • Phospholipases A2
  • Plant Proteins / immunology
  • Polysaccharides / analysis
  • Polysaccharides / immunology*
  • Xylose / analysis
  • Xylose / immunology*
  • beta-Fructofuranosidase

Substances

  • Antibodies
  • Bee Venoms
  • Enzymes, Immobilized
  • Epitopes
  • Glycoproteins
  • Plant Proteins
  • Polysaccharides
  • Fucose
  • Asparagine
  • Xylose
  • Horseradish Peroxidase
  • Phospholipases A
  • Phospholipases A2
  • Glycoside Hydrolases
  • beta-Fructofuranosidase