Genetic interrelationships of saccharolytic Clostridium botulinum types B, E and F and related clostridia as revealed by small-subunit rRNA gene sequences

FEMS Microbiol Lett. 1993 Mar 15;108(1):103-10. doi: 10.1111/j.1574-6968.1993.tb06081.x.


The phylogenetic interrelationships of saccharolytic C. botulinum types B, E and F together with eleven other saccharolytic clostridia were examined by 16S rRNA gene sequencing. Comparative analysis of the sequence data revealed that the saccharolytic C. botulinum types B, E and F were highly related and represent a single genetic group. Strains of C. barati and C. butyricum that produce botulinal neurotoxin revealed almost 100% 16S rRNA sequence identity with their respective non-toxigenic counterparts and were phylogenetically distinct from saccharolytic C. botulinum (types B, E and F). Proteolytic C. botulinum type F was shown to be phylogenetically remote from the saccharolytic C. botulinum group. The implications of the sequence data for the taxonomy of the C. botulinum complex are discussed.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Clostridium botulinum / classification
  • Clostridium botulinum / genetics*
  • DNA Probes
  • DNA, Bacterial / genetics
  • Molecular Sequence Data
  • Phylogeny
  • RNA, Bacterial / genetics*
  • RNA, Ribosomal, 16S / genetics*
  • Sequence Homology, Nucleic Acid
  • Species Specificity


  • DNA Probes
  • DNA, Bacterial
  • RNA, Bacterial
  • RNA, Ribosomal, 16S

Associated data

  • GENBANK/X68170
  • GENBANK/X68171
  • GENBANK/X68172
  • GENBANK/X68173
  • GENBANK/X68174
  • GENBANK/X68175
  • GENBANK/X68176
  • GENBANK/X68178
  • GENBANK/X68179
  • GENBANK/X68180
  • GENBANK/X68181
  • GENBANK/X68182
  • GENBANK/X68183
  • GENBANK/X68184