A Staphylococcus aureus-specific oligonucleotide probe derived from 16S rRNA gene sequences

Lett Appl Microbiol. 1993 Apr;16(4):203-6. doi: 10.1111/j.1472-765x.1993.tb01397.x.

Abstract

Genes encoding 16S rRNA were sequenced from 16 species of Staphylococcus. Sequence analysis highlighted a potential Staph. aureus-specific region and a complementary oligonucleotide probe was synthesized and its specificity tested. Northern blotting indicated molecular specificity, and dot blots to RNA from Staph. aureus, Staph. capitis, Staph. caprae, Staph. carnosus, Staph. caseolyticus, Staph. cohnii, Staph. epidermidis, Staph. gallinarum, Staph. haemolyticus, Staph. hominis, Staph. hyicus, Staph. saprophyticus, Staph. sciuri, Staph. simulans, Staph. warneri and Staph. xylosus indicated species-specificity.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Composition
  • Base Sequence
  • Blotting, Northern
  • Genes, Bacterial
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Oligonucleotide Probes* / chemistry
  • RNA, Bacterial / chemistry
  • RNA, Bacterial / genetics*
  • RNA, Ribosomal, 16S / genetics*
  • RNA, Ribosomal, 16S / isolation & purification
  • Sequence Alignment
  • Sequence Homology, Nucleic Acid
  • Species Specificity
  • Staphylococcus / genetics
  • Staphylococcus aureus / genetics*
  • Staphylococcus aureus / isolation & purification

Substances

  • Oligonucleotide Probes
  • RNA, Bacterial
  • RNA, Ribosomal, 16S